PROTEOBACTERIA >> Alpha >> Acetobacter >> Acetobacter malorum
ACETOBACTER MALORUM
| Class: | Alpha Proteobacteria |
| Genus: | Acetobacter |
| Species: | Acetobacter malorum |
| Description: | The identification and quantification of Acetobacter malorum and Acetobacter cerevisiae in wine and vinegar were performed using the Real-Time PCR (RT-PCR) with two TaqMan-MGB probes designed to amplify the internal transcribed spacer (ITS) region between the 16S-23S rRNA genes. The primers and probes were highly specific, with a detection limit of 10² cells/ml for both species, and the efficiency of the technique was >80%. The RT-PCR technique with these two new TaqMan-MGB probes, together with the five (Acetobacter aceti, Acetobacter pasteurianus, Gluconobacter oxydans, Gluconacetobacter hansenii and Gluconacetobacter europaeus) that are already available (Torija et al., 2010), were validated on known concentrations of Acetic Acid Bacteria (AAB) grown in glucose medium (GY) and in inoculated matrices of wine and vinegar. Furthermore, this technique was applied to evaluate the AAB population in real wine samples collected in the Canary Islands. |
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| Genbank: | NZ_LHZC00000000.1 [Genome] [Nucleotide] |
| Size (Mb): | 3.83 |
| GC %: | 57.15 |
| Genes: | 3,453 |
| CDS Number: | 3,390 |
| Reference: | Valera MJ1, Torija MJ, Mas A, Mateo E."Acetobacter malorum and Acetobacter cerevisiae identification and quantification by Real-Time PCR with TaqMan-MGB probes."Food Microbiol. 2013 Oct;36(1):30-9. doi: 10.1016/j.fm.2013.03.008. Epub 2013 Apr 13. |
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